Crk anticorps (pTyr221)

N° du produit ABIN498832

Cet anticorps Lapin Polyclonal détecte spécifiquement Crk dans IHC (p). Il présente une réactivité envers Humain, Souris et Rat.

Aperçu rapide pour Crk anticorps (pTyr221) (ABIN498832)

Antigène: Crk (CRK) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian) (CRK))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Crk est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-Crk anticorps

Épitope: pTyr221

Specificité: This antibody detects endogenous levels of p-Crk2 protein only when phosphorylated at Try221.

Réactivité croisée (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Pureté: > 95 % by SDS-PAGE

Information d'application

Indications d'application: Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur Crk

Antigène: Crk (CRK) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian) (CRK))

Autre désignation: CRK

Sujet: The Crk family of adapter proteins including Crk II, Crk I and Crk L consist mostly of SH2 and SH3 domains. Through the interactions between SH2 domain and phosphotyrosine residues and/or between SH3 domain and proline- rich motifs, they are involved in a variety of signaling cascades. Crk I and Crk II are encoded by the same gene, which undergoes alternative splicing to yield these two proteins, but differ in their biological activities. Crk II has less transforming activity than Crk I, although both Crk I and Crk II bind to many tyrosine-phosphorylated proteins that bind to GRB2. In addition, Crk II becomes rapidly tyrosine-phosphorylated in response to stimulation with insulin-like growth factor-I (IGFI) and might be involved in the IGF-I receptor signalling pathway. The gene encoding Crk I and II maps to human chromosome 17p13, a region which demonstrates frequent deletion or loss of heterozygosity in a wide range of human cancers.Synonyms: Adapter molecule crk, Proto-oncogene C-crk, p38

Poids moléculaire: approx. 42 kDa

ID gène: 1398

NCBI Accession: NP_005197

UniProt: P46108

Pathways: Neurotrophin Signaling Pathway, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor